Cultivation in Vitro of Malignant Tumors

The growth of malignant tumors in cultures outside the body has been studied in a large number of experiments. The technique employed has already been described 1 and need not be repeated here. W e propose, in this article, to describe the growth characteristics of the different varieties of tumors employed for cultivation. The tumors employed were as follows: the Rous chicken sarcoma (2) , the Ehrlich and the Jensen rat sarcomata, the FlexnerJobling rat carcinoma, a dog carcinoma, and human sarcomata (3) and carcinomata. By far the largest number of tests was made with the Rous chicken sarcoma, of which nearly four hundred cultures were studied. The next largest number of tests was made in connection with the malignant human tumors, of which nearly two hundred cultures were made. Many cultures were made of the other varieties.